Mycoplasma Test Sample Submission

Sample Preparation Instructions

1. We receive both cell culture media and genomic DNA samples for this test.

  • 1a. For cell culture media samples, sample media must be obtained from cell culture that has been grown for at least 72 hours with 80% – 95% confluence. Centrifuge at 2000g for 3 minutes to pellet cells and submit 1ml of cell culture media in 1.5ml microcentrifuge tubes.
  • 1b. For genomic DNA samples, at least 20 μl genomic DNA with a concentration of more than 50 ng/μl can be provided for detection.

2. Clearly label Sample Name, Person Name, and Submission Date on both the cap and the side of the tube with a blue or black permanent marker. (Samples can be frozen at -20 °C for up to 1 week before submission, but prevent freeze/thaw cycle.)

3. Please fill out the sample submission form. External users please ship the samples to below address. University users please email TEP-service@illinois.edu to schedule a time to drop the samples at the entrance of south parking lot of Mills Breast Cancer Institute.

Hui Xu

3rd floor, Mills Breast Cancer Institute

509W University Ave

Urbana, IL 61801

 

Note:

TEP does not accept cultures infected with HIV or Biosafety Level 3 or 4 agents. To the best of knowledge, the cell line being submitted should be free of hazardous materials, agents, and carcinogens.

 

Sample Submission

Please submit orders on the online ordering system FBS.

First-time user, please email TEP-service@illinois.edu to have an account set up.