Mycoplasma Test Sample Submission

Sample Preparation Instructions

1. We receive both cell culture media and genomic DNA samples for this test.

  • 1a. For cell culture media samples, sample media must be obtained from cell culture that has been grown for at least 72 hours with 80% – 95% confluence. Centrifuge at 2000g for 3 minutes to pellet cells and submit 1ml of cell culture media in 1.5ml microcentrifuge tubes.
  • 1b. For genomic DNA samples, at least 20 μl genomic DNA with a concentration of more than 50 ng/μl can be provided for detection.

2. Clearly label Sample Name, Person Name, and Submission Date on both the cap and the side of the tube with a blue or black permanent marker. (Samples can be frozen at -20 °C for up to 1 week before submission, but prevent freeze/thaw cycle.)

3. Please fill out the sample submission form. External users please ship the samples to below address. University users please email TEP-service@illinois.edu to schedule a time to drop the samples at the entrance of south parking lot of Mills Breast Cancer Institute.

Hui Xu

405 N. Mathews,

Urbana, IL 61801

 

Note:

TEP does not accept cultures infected with HIV or Biosafety Level 3 or 4 agents. To the best of knowledge, the cell line being submitted should be free of hazardous materials, agents, and carcinogens.

 

Sample Submission

Please submit orders on the online ordering system FBS.

First-time user, please email TEP-service@illinois.edu to have an account set up.