Seahorse XFe96 Analyzers measure OCR and ECAR of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
Seahorse XFe96 Analyzer
The Seahorse Analyzer provides non-invasive measurement with no addition of dyes, labels or reporters. It measures adherent cells without requiring trypsinization, and suspension cells may be measured using Cell-Tak® cell and tissue attachment medium. It typically requires only 5×103 to 5×105 per 96-well Seahorse assay.
The equipment is compatible with convenient, easy-to-use Seahorse XF test kits and other Seahorse XF reagents. It simplifies the study of cellular metabolism by providing pre-calibrated, pre-tested reagents for measuring metabolic phenotype, mitochondrial respiration, glycolysis, and fatty acid oxidation. OCR and ECAR rate are calculated in 2-5 minutes using data acquired every 14 seconds. Measurements may be repeated indefinitely to measure kinetic responses. Up to four test compounds may be added automatically to each well during the assay. Measurements may be performed before and after each compound is added.
Perform Cell Mito Stress Test in the Presence of Drug X (UK5099)
Seahorse Analyzer training and protocols
Please find more information on this link: Seahorse XF Assay Learning Center
- Let us know your protocol to prepare plates and reagent, also reserve the equipment
- Submit samples
- Run the experiment and provide a report
|Items||Price per plate|
|Cell seeding, including labor, Seahorse XF96 V3 PS Culture Microplates and other consumables. Media will be provided by users.||$50|
|Seahorse XF96 V3 PS Culture Microplates||$13|
|Cell washing, including labor, Seahorse assay media and other consumables||$100|
|Cartridge hydration and reagent loading, including labor, Seahorse XFe96 FluxPak mini cartridge, calibrant and other consumables. Assay kit will be provided by user.||$150|
|Seahorse analyzer operated by TEP||$295|